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SPIDER
(System for
Processing Image
Data from
Electron microscopy and
Related fields) is an
image processing system for electron microscopy.
- News:
- SPIDER is an open source project maintained by unpaid volunteers.
- Emphasis on:
- Features:
- History:
- Originated in 1978 by
Joachim Frank who
shared the 2017 Nobel Prize in Chemistry for his pioneering cryo-em
research done at the
Wadsworth Center in Albany, NY.
- Contributors (in chronological order):
J. Frank,
B. Shimkin,
H. Dowse,
L. Miranda,
C. Mannella,
J. P. Bretaudiere,
M. V. Heel,
A. Verschoor,
M. Radermacher,
A. Leith,
J. M. Carazo,
P. Penczek,
S. Sibal,
L. Odesanya,
Y. H. Li,
M. Ladjadj,
Y. Chen,
K. R. Lata,
J. Zhu,
W. P. Liu,
B. Rath,
C. Yang,
B. Baxter,
R. Hegerl,
A. Frangakis,
L. Joyeux,
Z. Huang,
R. J. Renka,
T. Shaikh,
J. Sengupta,
J. LeBarron,
N. Boisset,
H. Gao,
G. Kishchenko,
J. M. Kennedy.
In addition SPIDER is dependent on the Fourier package
FFTW by
M Frigo and SG Johnson.
- Maintained since 2007 by: ArDean Leith & Tanvir Shaikh.
During these 11 years they added numerous operations, procedures, parallelizations,
and over 500 pages of documentation. There have been no significant contributors
other than Leith, Shaikh, Kischenko, and Kennedy since 2007.
- Spotlight:
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Spherical Deconvolution Improves Quality of Single Particle Reconstruction.
Kishchenko GP and Leith A. J. Struct. Biol., 187, 84-92 (2014).
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Use of SPIDER and SPIRE in Image Reconstruction .
Leith A, Baxter AW and Frank J.
International Tables for Crystallography.
Vol. F, ch. 19.8, 620-623 (2012)
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SPIDER image processing for single-particle reconstruction of
biological macromolecules from electron micrographs.
Nature Protocols 3, 1941-1974 (2008)
Download tar archives of procedures.
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SPIDER and Web: Processing and Visualization of Images in 3D
Electron Microscopy and Related Fields.
Frank J, Radermacher M, Penczek P, Zhu J, Li Y, Ladjadj M, and Leith A.
J. Struct. Biol. 116: 190-199 (1996)
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