|AT WN||[Automatic particle selection]|
|AT SA||[Automatic particle picking - statistical parameters ||]|
|AT IT||[Automatic particle picking - sort doc file]|
.PIXEL NEIGHBORHOOD FOR SEARCH: 13
[Enter the number of pixels which corresponds approximately to the size of the particle. For the above input of: 13, the filtered image is searched to find the highest peak in a 13 by 13 square. In this way picking the same particle is avoided and also the nearest neighbour distance is taken into account. Must be >= 3.]
.CC THRESHOLD FOR PEAKS: 0.7
[Enter the threshold value i.e., only peaks having correlation coefficients over this value are picked up.]
.MICROGRAPH EDGE DIMENSIONS: 80, 80
[Peaks closer to the edge of the micrograph are excluded. i.e.,in the example if X1 and Y1 are the X- and Y- coordinates of the peaks, and nxe, nye, are the dimensions of the micrograph edge, and nx and sy are the dimensions of the micrograph, then the following conditions are met:
(X1-(nxe/2+1)) is >= to 1.
(X1+(nxe/2+1)) is <= to nx.
(Y1-(nye/2+1)) is >= to 1.
(Y1+(nye/2+1)) is <= to ny.
.PEAK LOCATION DOC FILE: DOC001
[Enter the name of the document file where you want the peaks to be stored. The peak positions (relative to origin) will be printed in the document file as follows: