LO I - Initialize files for Locating motif inside volume

(8/04/2008)

PURPOSE

Create FFT of search volume, FFT of squared search volume, and FFT of rotated padded mask, as requested. These files are needed for: 'LO LSD' and 'LO'.

SEE ALSO

LO LSD [Prepare LSD volume for Locating motif inside volume]
LO [Locate motif inside a search volume]

USAGE

.OPERATION: LO I

.CREATE SEARCH FFT'S (S), MASK FFT (M), OR BOTH (B): B
[Select type of output files to be created. For a motif with a rotationally invariant mask, both types of output can be created initially before the motif is rotated. For a rotationally varying mask the mask fft must be recalculated at each orientation.]

.SEARCH VOLUME FILE: BIG000
[For efficiency this volume must be padded, if necessary, to even number of rows and slices before the operation can be used.]

If answer to initial question was 'S' or 'B' then following two queries appear:

.SEARCH VOLUME FFT OUTPUT FILE: search_fft
[Enter name for FFT of search volume.]

.SEARCH VOLUME SQ. FFT OUTPUT FILE: search_sq_fft
[Enter name for FFT of search volume squared.]

If answer to initial question was 'M' or 'B' then following two queries appear:

.ROTATED MOTIF MASK INPUT FILE: rot_mask
[Enter name of rotated motif mask.]

.PADDED MOTIF MASK FFT OUTPUT FILE: mask_fft
[Enter name for padded rotated motif mask FFT file.]

NOTES

  1. The following procedures:
    sigs_settings.spi, sigs_pub.spi, and sigsloop.spi for an example of usage.

  2. For futher info see:
    RAMOS (RApid MOtif Search) / Signature Search / Docking

  3. References:
    B.K. Rath et al. (2003) Journal of Structural Biology 144, 95-103.
    Alan Roseman (2003) Ultramicroscopy 94, 225-236.

SUBROUTINES

MOTIF_INIT

CALLER

MOTIF1

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