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SPIDER
(System for
Processing Image
Data from
Electron microscopy and
Related fields) is an
image processing system for electron microscopy.
- News:
- SPIDER is currently an open source project maintained by volunteers.
It has been in use for 42 years and for 2020 it has been modified to
accept MRC format files. This is probably the final
release. The last release will continue to be available at:
github.com/spider-em/SPIDER
- Emphasis on:
- Features:
- History:
- Originated in 1978 by
Joachim Frank who
shared the 2017 Nobel Prize in Chemistry for his pioneering cryo-em
research done at the
Wadsworth Center in Albany, NY.
- Contributors (in chronological order):
J. Frank,
B. Shimkin,
H. Dowse,
L. Miranda,
W. Goldfarb,
S. Basu,
R. Banerjee,
C. Mannella,
J. P. Bretaudiere,
M. V. Heel,
A. Verschoor,
M. Radermacher,
A. Leith,
J. M. Carazo,
P. Penczek,
S. Sibal,
L. Odesanya,
Y. H. Li,
M. Ladjadj,
Y. W. Chen,
K. R. Lata,
J. Zhu,
W. P. Liu,
B. Rath,
C. Yang,
B. Baxter,
R. Hegerl,
A. Frangakis,
L. Joyeux,
Z. Huang,
R. J. Renka,
T. Shaikh,
J. Sengupta,
J. LeBarron,
N. Boisset,
H. Gao,
G. Kishchenko,
J. M. Kennedy,
A. Gunggong.
In addition SPIDER is dependent on the Fourier package
FFTW by
M Frigo and SG Johnson.
- Maintained since 2007 by: ArDean Leith & Tanvir Shaikh.
During this time they added numerous operations, procedures, parallelizations,
and over 500 pages of documentation. Since 2007 here have been no significant
contributors other than Leith, Shaikh, Kishchenko, and Kennedy.
- Spotlight:
-
Spherical Deconvolution Improves Quality of Single Particle Reconstruction.
Kishchenko GP and Leith A. J. Struct. Biol., 187, 84-92 (2014).
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Use of SPIDER and SPIRE in Image Reconstruction .
Leith A, Baxter AW and Frank J.
International Tables for Crystallography.
Vol. F, ch. 19.8, 620-623 (2012)
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SPIDER image processing for single-particle reconstruction of
biological macromolecules from electron micrographs.
Nature Protocols 3, 1941-1974 (2008)
Download tar archives of procedures.
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SPIDER and Web: Processing and Visualization of Images in 3D
Electron Microscopy and Related Fields.
Frank J, Radermacher M, Penczek P, Zhu J, Li Y, Ladjadj M, and Leith A.
J. Struct. Biol. 116: 190-199 (1996)
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